Expressing:Article Title: Nicking Activity of M13 Bacteriophage Protein 2
Article Snippet: .. The P2 expression vector was constructed using the ligation-independent cloning (LIC) N-terminal fusion plasmid, pET His6 GFP TEV LIC cloning vector (p1GFP) (Addgene Plasmid #29663), which carries the green fluorescent protein (GFP) gene and the 6× histidine tag at the N terminus of the protein and is expressed as His6X-GFP-TEV-P2 (P2-GFP). .. Vector DNA was digested with SspI Fast Digest Restriction Enzyme (#R0132S, Thermo Fisher Scientific, Waltham, MA, USA) and then purified via agarose gel electrophoresis using a Gel DNA Recovery Kit (Zymo-Research, Orange, CA, USA).
Article Title: Nicking Activity of M13 Bacteriophage Protein 2.
Article Snippet: .. The P2 expression vector was constructed using the ligation-independent cloning (LIC) N-terminal fusion plasmid, pET His6 GFP TEV LIC cloning vector (p1GFP) (Addgene Plasmid #29663), which carries the green fluorescent protein (GFP) gene and the 6× histidine tag at the N terminus of the protein and is expressed as His6X-GFP-TEV-P2 (P2-GFP). .. Vector DNA was digested with SspI Fast Digest Restriction Enzyme (#R0132S, Thermo Fisher Scientific, Waltham, MA, USA) and then purified via agarose gel electrophoresis using a Gel DNA Recovery Kit (Zymo-Research, Orange, CA, USA).
Plasmid Preparation:Article Title: Nicking Activity of M13 Bacteriophage Protein 2
Article Snippet: .. The P2 expression vector was constructed using the ligation-independent cloning (LIC) N-terminal fusion plasmid, pET His6 GFP TEV LIC cloning vector (p1GFP) (Addgene Plasmid #29663), which carries the green fluorescent protein (GFP) gene and the 6× histidine tag at the N terminus of the protein and is expressed as His6X-GFP-TEV-P2 (P2-GFP). .. Vector DNA was digested with SspI Fast Digest Restriction Enzyme (#R0132S, Thermo Fisher Scientific, Waltham, MA, USA) and then purified via agarose gel electrophoresis using a Gel DNA Recovery Kit (Zymo-Research, Orange, CA, USA).
Article Title: Nicking Activity of M13 Bacteriophage Protein 2.
Article Snippet: .. The P2 expression vector was constructed using the ligation-independent cloning (LIC) N-terminal fusion plasmid, pET His6 GFP TEV LIC cloning vector (p1GFP) (Addgene Plasmid #29663), which carries the green fluorescent protein (GFP) gene and the 6× histidine tag at the N terminus of the protein and is expressed as His6X-GFP-TEV-P2 (P2-GFP). .. Vector DNA was digested with SspI Fast Digest Restriction Enzyme (#R0132S, Thermo Fisher Scientific, Waltham, MA, USA) and then purified via agarose gel electrophoresis using a Gel DNA Recovery Kit (Zymo-Research, Orange, CA, USA).
Construct:Article Title: Nicking Activity of M13 Bacteriophage Protein 2
Article Snippet: .. The P2 expression vector was constructed using the ligation-independent cloning (LIC) N-terminal fusion plasmid, pET His6 GFP TEV LIC cloning vector (p1GFP) (Addgene Plasmid #29663), which carries the green fluorescent protein (GFP) gene and the 6× histidine tag at the N terminus of the protein and is expressed as His6X-GFP-TEV-P2 (P2-GFP). .. Vector DNA was digested with SspI Fast Digest Restriction Enzyme (#R0132S, Thermo Fisher Scientific, Waltham, MA, USA) and then purified via agarose gel electrophoresis using a Gel DNA Recovery Kit (Zymo-Research, Orange, CA, USA).
Article Title: Nicking Activity of M13 Bacteriophage Protein 2.
Article Snippet: .. The P2 expression vector was constructed using the ligation-independent cloning (LIC) N-terminal fusion plasmid, pET His6 GFP TEV LIC cloning vector (p1GFP) (Addgene Plasmid #29663), which carries the green fluorescent protein (GFP) gene and the 6× histidine tag at the N terminus of the protein and is expressed as His6X-GFP-TEV-P2 (P2-GFP). .. Vector DNA was digested with SspI Fast Digest Restriction Enzyme (#R0132S, Thermo Fisher Scientific, Waltham, MA, USA) and then purified via agarose gel electrophoresis using a Gel DNA Recovery Kit (Zymo-Research, Orange, CA, USA).
Ligation:Article Title: Nicking Activity of M13 Bacteriophage Protein 2
Article Snippet: .. The P2 expression vector was constructed using the ligation-independent cloning (LIC) N-terminal fusion plasmid, pET His6 GFP TEV LIC cloning vector (p1GFP) (Addgene Plasmid #29663), which carries the green fluorescent protein (GFP) gene and the 6× histidine tag at the N terminus of the protein and is expressed as His6X-GFP-TEV-P2 (P2-GFP). .. Vector DNA was digested with SspI Fast Digest Restriction Enzyme (#R0132S, Thermo Fisher Scientific, Waltham, MA, USA) and then purified via agarose gel electrophoresis using a Gel DNA Recovery Kit (Zymo-Research, Orange, CA, USA).
Article Title: Nicking Activity of M13 Bacteriophage Protein 2.
Article Snippet: .. The P2 expression vector was constructed using the ligation-independent cloning (LIC) N-terminal fusion plasmid, pET His6 GFP TEV LIC cloning vector (p1GFP) (Addgene Plasmid #29663), which carries the green fluorescent protein (GFP) gene and the 6× histidine tag at the N terminus of the protein and is expressed as His6X-GFP-TEV-P2 (P2-GFP). .. Vector DNA was digested with SspI Fast Digest Restriction Enzyme (#R0132S, Thermo Fisher Scientific, Waltham, MA, USA) and then purified via agarose gel electrophoresis using a Gel DNA Recovery Kit (Zymo-Research, Orange, CA, USA).
Cloning:Article Title: Nicking Activity of M13 Bacteriophage Protein 2
Article Snippet: .. The P2 expression vector was constructed using the ligation-independent cloning (LIC) N-terminal fusion plasmid, pET His6 GFP TEV LIC cloning vector (p1GFP) (Addgene Plasmid #29663), which carries the green fluorescent protein (GFP) gene and the 6× histidine tag at the N terminus of the protein and is expressed as His6X-GFP-TEV-P2 (P2-GFP). .. Vector DNA was digested with SspI Fast Digest Restriction Enzyme (#R0132S, Thermo Fisher Scientific, Waltham, MA, USA) and then purified via agarose gel electrophoresis using a Gel DNA Recovery Kit (Zymo-Research, Orange, CA, USA).
Article Title: Nicking Activity of M13 Bacteriophage Protein 2.
Article Snippet: .. The P2 expression vector was constructed using the ligation-independent cloning (LIC) N-terminal fusion plasmid, pET His6 GFP TEV LIC cloning vector (p1GFP) (Addgene Plasmid #29663), which carries the green fluorescent protein (GFP) gene and the 6× histidine tag at the N terminus of the protein and is expressed as His6X-GFP-TEV-P2 (P2-GFP). .. Vector DNA was digested with SspI Fast Digest Restriction Enzyme (#R0132S, Thermo Fisher Scientific, Waltham, MA, USA) and then purified via agarose gel electrophoresis using a Gel DNA Recovery Kit (Zymo-Research, Orange, CA, USA).
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